PySupercharge Example

Green Fluorescent Protein (PDB 1GFL)

LCD Graph

Insert Protein Amino Acid Sequence
(Inserted: GFP Sequence)

Threshold

Window Size

Target AA

Substitute AA

Exclusion

e.g. 45, 127

Histidine Charge

AvNAPSA Threshold (Max)

Protein's PDB File
1gfl_monomer.pdb

Consurf 'consurf_grades.txt' or '.grades' file
gfp-consurf.grades